Journal: Frontiers in Endocrinology

Article Title: Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society

doi: 10.3389/fendo.2020.00065

Figure Lengend Snippet: (A) Distal-proximal representative images in Hematoxylin & Eosin (H&E) stain of 4μm paraffin sections of femur, tibia and tail of 8-week-old mice. Note that the artefactually empty region in the center of tibia and femur corresponds to the expansion of the central vein lumen due to fixation-mediated retraction. (B) Left panels: Murine bone marrow adipocytes in the tail vertebrae of 24-week-old mice (cBMAT) of 3μm paraffin sections stained with H&E (top) and 6μm sections stained with von Kossa/Methylene Blue (bottom). Right panels: murine bone marrow adipocytes in the proximal tibia of 24 weeks-old mice (rBMAT) of 3μm paraffin sections stained with H&E (top) and 100μm sections stained with perilipin immunofluorescence (bottom, Perilipin in green and TO-PRO-3 nuclear counterstain) of 50-week-old mice. All images correspond to C57BL/6 female mice housed at room temperature fed ad libitum standard diet. BM, bone marrow; BMAd, bone marrow adipocyte; CB, cortical bone; cBMAT, constitutive bone marrow adipose tissue; H&E, hematoxylin and eosin; rBMAT, regulated bone marrow adipose tissue.

Article Snippet: Acute fasting (48 h) , Sprague-Dawley rats , BMAd size: ↓proximal tibia ↔tail vertebrae (O, μCT) , ( ) .

Techniques: Staining, Immunofluorescence

Journal: Frontiers in Endocrinology

Article Title: Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society

doi: 10.3389/fendo.2020.00065

Figure Lengend Snippet: Challenges and goals ahead for the BMA field and the BMAS WG in methodologies.

Article Snippet: Acute fasting (48 h) , Sprague-Dawley rats , BMAd size: ↓proximal tibia ↔tail vertebrae (O, μCT) , ( ) .

Techniques: In Vivo, Extraction, Comparison, Imaging, Isolation, Functional Assay, Control

Journal: Frontiers in Endocrinology

Article Title: Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society

doi: 10.3389/fendo.2020.00065

Figure Lengend Snippet: List of abbreviations.

Article Snippet: Acute fasting (48 h) , Sprague-Dawley rats , BMAd size: ↓proximal tibia ↔tail vertebrae (O, μCT) , ( ) .

Techniques: Computed Tomography, Binding Assay, Staining, Electron Microscopy, Fluorescence, FACS, Spectroscopy, Virus, Immunohistochemistry, Magnetic Resonance Imaging, Polymerase Chain Reaction, Derivative Assay, Recombinant, Red Blood Cell Lysis, Real-time Polymerase Chain Reaction, Serial Time-encoded Amplified Microscopy, Imaging

Journal: Frontiers in Endocrinology

Article Title: Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society

doi: 10.3389/fendo.2020.00065

Figure Lengend Snippet: In vivo modulation of bone marrow adipose tissue by dietary and environmental factors.

Article Snippet: Acute fasting (48 h) , Sprague-Dawley rats , BMAd size: ↓proximal tibia ↔tail vertebrae (O, μCT) , ( ) .

Techniques: In Vivo, Animal Model

Journal: NPJ Biofilms and Microbiomes

Article Title: Irrigation of the intramedullary channel improves outcome of DAIR in a sheep model

doi: 10.1038/s41522-024-00643-0

Figure Lengend Snippet: A RIL over 13 weeks of the infected animals from the delayed group ( n = 5). B correlation of RIL and CFU at revision ( n = 5). C Correlation of RIL and CFU at euthanasia. D Evaluation of bone volume and E bone density within the 2 mm of osteotomy. Individual data represented including median value and range. Statistical analyses were performed using Dunn’s multiple comparisons test. F Correlation of bone volume and bone density data obtained from the clinical CT with sensor data at 5 weeks and at G . 8 weeks. Spearman correlation values ( r ) are indicated in the correlation graphs.

Article Snippet: A bone mineral density (BMD) calibration phantom (QRM-BDC/6, QRM GmbH, Möhrendorf, Germany) was present in all scans to be able to calibrate the image data from HU to BMD values.

Techniques: Infection

Journal: NPJ Biofilms and Microbiomes

Article Title: Irrigation of the intramedullary channel improves outcome of DAIR in a sheep model

doi: 10.1038/s41522-024-00643-0

Figure Lengend Snippet: A RIL over 11 weeks of the 3 weeks infected ( n = 5, black) and 3 weeks + IML ( n = 2, red) animals. B , C Correlation of RIL and CFU at revision and at euthanasia, respectively. D , E Evaluation of bone volume and bone density within the 2 mm of osteotomy in the 3 weeks and 3 weeks + IML groups. Individual data represented including median value and range. Statistical analyses using 2-way ANOVA with Šídák’s multiple comparisons test. Spearman correlation values ( r ) are indicated in the correlation graphs.

Article Snippet: A bone mineral density (BMD) calibration phantom (QRM-BDC/6, QRM GmbH, Möhrendorf, Germany) was present in all scans to be able to calibrate the image data from HU to BMD values.

Techniques: Infection